What are TLC plates made of?

by Mildred Parker | views: 354

The thin-layer chromatography plate is composed of silica gel (the adsorbent ) adhered to a plastic backing for support. Reminder: silica is a polar compound.

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Additionally, are tlc plates made of glass?

Plates (Stationary Phase)

The backing of TLC plates is often composed of glass, aluminum, or plastic.

Additionally, you might ask, how do you make a tlc plate? TLC Slurry preparation for soft layer coating: Silica Gel and small amount of calcium sulfate (gypsum) is mixed with water. This mixture is spread as a thick slurry on a clean glass plate. The resultant plate is dried and activated by heating in an oven for thirty minutes at 110°C.

That may lead you to ask, do tlc plates have silica gel? The silica gel remains the most important adsorbent for TLC separation. The kinetic properties of the silica gel thin-layer and the new TLC plates have been presented. Other materials used as stationary phase were alumina, zirconium oxide, Florisil, and ion-exchanger.

What is a TLC plate?

Thin layer chromatography (TLC) is a widely used separation technique for quantitative and qualitative analysis. It uses a thin layer of a stationary phase coated on a glass, plastic, or aluminum plate. A liquid solvent called the mobile phase carries the sample and separates it as it moves across the plate.

29 Related Questions & Answers

How do I select a solvent for TLC?

The choice of solvent or a mixture of solvents used in TLC is solely guided by two important factors : (a) the nature of the constituent to be separated i.e., whether it is polar or non-polar ; and (b) the nature of the process involved i.e., whether it is a case of 'adsorption' or 'partition chromatography'.

Why is ethyl acetate a good solvent for TLC?

Ethyl acetate is a polar solvent, but it is often used in TLC. TLC's stationary phase is usually silica gel, which is polar. I thought the whole point of TLC is to separate the polar compounds (travels less, low Rf) and nonpolar compounds (travels farther, high Rf).

Which materials will you use to prepare the TLC development chamber?

An inexpensive chamber can be made using a beaker and watch glass (Figure 2.23b). Cut a piece of filter paper (or two) so that when placed in the chamber, the filter paper fits inside the chamber and is flat on the bottom but not obscuring your view of the inside (Figure 2.23c).

How do you make slurry silica?

In a beaker, measure out the required amount of silica or alumina. In a separate flask or beaker, measure solvent approximately one and a half times the volume of silica. Add the silica to the solvent, a little at a time, while swirling. Use a Pasteur pipette or glass rod to mix the slurry.

What is silica gel G?

A binder Gypsum (G) is added to this grade of Silica Gel which helps the Silica Gel to firmly settle on the TLC sheet. This grade also assures better Rf value for the compounds which get separated. Making it easy to distinguish between very closely related compounds.

What other materials can be used instead of silica gel in TLC?

The silica gel remains the most important adsorbent for TLC separation. The kinetic properties of the silica-gel thin layer and the new TLC plates have been presented. Other materials used as stationary phase were alumina, zirconium oxide, Florisil, and ion-exchanger.

Is silica gel a binder?

The TLC silica gel 60 G plates consist of silica gel having a pore size of 60 Å as the adsorption formulation, with gypsum as a binder material.

How do you make a chromatogram?

  • Spots of ink or plant dye are placed on a pencil line.
  • As the paper is lowered into the solvent, some of the dye spreads up the paper.
  • The paper has absorbed the solvent, and the dye has spread further up the paper.
  • What is a solvent front in TLC?

    In chromatography, the solvent front is the position on the TLC plate indicating the furthest distance traveled by the developing solvent (or eluent)

    Which method is best for the preparation of TLC plate?

    TLC plates are usually commercially available, with standard particle size ranges to improve reproducibility. They are prepared by mixing the adsorbent, such as silica gel, with a small amount of inert binder like calcium sulfate (gypsum) and water.

    How can you tell if a plate is TLC?

    Is methanol a good general solvent for TLC?

    Is methanol a good general solvent for TLC? Methanol is not suitable. I am a bit surprised why are you switching to methanol when you got a good separation with ethyl acetate.

    What makes a good TLC solvent?

    As a rule of thumb, your sample solvent should: 1) dissolve your sample when cold, 2) have a low boiling point (so it will evaporate quickly) and 3) be as non-polar as possible (e.g., dichloromethane), because if some sample solvent should remain on a developing TLC plate, it will not greatly change the polarity of the ...

    What is the best solvent for chromatography?

    SolventPolarity (arbitrary scale of 1-5)Suitability
    Water1 – Most polarGood
    Rubbing alcohol (ethyl type) or denatured alcohol2 – High polarityGood
    Rubbing alcohol (isopropyl type)3 – Medium polarityGood
    Vinegar3 – Medium polarityGood

    Why methanol is used in TLC?

    Methanol is a polar protic solvent which also can act as a nucleophile. Ethyl acetate is a polar aprotic solvent, non-nucleophilic in nature. There is a possibility that your compound might have reacted with metanol.

    Which solvent is best for recrystallization?

    Methanol; A recrystallization needs to dissolve the compound only near its boiling point. Also, the recrystallization should not melt the compound. Therefore, the best solvent is methanol.